Linkage mapping of Lentil (Lens culinaris L.) genome using recombinant inbred lines revealed by AFLP, ISSR, RAPD and some morphologic markers

Abstract

We have constructed a molecular linkage map of Lentil (Lens culinaris L.) in a RIL population (developed from the parents Precoz x WA8949041) of 94 plants with 166 markers. The resulting linkage map consists of 11 (varying between 50.9–436.5 cM) linkage groups covering 1396.3cM with an average map distance between framework markers of 8.4 cM. Most of the RAPD and ISSR markers
were evenly distributed across the genome. By using 40 primer combinations, we were able to generate 391 AFLP markers in the RIL
population. The majority of the AFLP markers clustered in linkage group 2. In the segregation distortion analysis, 185 markers deviated
from Mendelian segregation. Of the 185 markers with skewed segregation, 108 markers were unlinked and the rest of the distorted
markers mapped to linkage groups. LG2 contained the most distorted marker among the linkage groups.